Mouse/Rat TGF beta 1 Protein (Animal-Free)
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| 产品名称 | Mouse/Rat TGF beta 1 Protein (Animal-Free) |
|---|---|
| 中文名称 | 重组小鼠/大鼠转化生长因子β1（无动物源） |
| 种属 | Mouse/Rat |
| 表达宿主 | E. coli |
| 纯度 | >98% as determined by SDS-PAGE. |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measure by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The ED₅₀ for this effect is <0.1 ng/mL. |
| 基因编号 | 7040 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P04202/entry |
| 别名 | Transforming growth factor beta 1, Differentiation inhibiting factor, Cartilage-inducing factor, Tgfb-1 |
| 蛋白序列 | MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS with polyhistidine tag at the C-terminus. |
| 蛋白标签 | C-His |
| 预测分子量 | The protein has a calculated MW of 13.8 kDa.The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis). |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. |
| 使用方式 | 1. Before opening, centrifuge at 3000 rpm for 5 mins. 2. Initial Reconstitution · Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL. · Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. · Store at 2°C to 8°C for up to 1 week. 3. Extended Storage · After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml. · Prepare aliquots (≥20 μl). · Store at -20°C or -80°C for 3 to 6 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | The product is shipped at Blue Ice |
| 储存条件 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 产品背景 | The transforming Growth Factors beta (TGF beta) family of cytokines are ubiquitous, multifunctional, and essential to survival. They play the central roles in growth, development, inflammation, repair, and host immunity. The mammalian TGF beta isoforms (TGF beta 1, beta 2 and beta 3) are secreted as potential precursors and possess a variety of cell surface receptors and produces at least two mediate signal transductions. |
*本产品仅供科研试验用，不可直接用于人体。
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