| 产品名称 | Human FGF-4 protein (Animal-Free) |
|---|---|
| 中文名称 | 重组人成纤维细胞生长因子4（无动物源） |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | >98 % as determined by SDS-PAGE |
| 内毒素 | < 0.1 EU per μg of the protein as determined by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using NIH/3T3 cells. The ED50for this effect is 0.08 ng/mL |
| 基因编号 | 2249 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P08620/entry |
| 别名 | HST-1, Transforming protein KS3, HBGF-4 |
| 蛋白序列 | APTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL |
| 蛋白标签 | N-His |
| 预测分子量 | The recombinant Human FGF-4 consists of 182 amino acids and predicts a molecular mass of 20.7 kDa. |
| 产品形式 | Lyophilized from PBS, pH 8.0. |
| 使用方式 | Lyophilized protein product should be stored desiccated below-18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20℃ |
| 产品背景 | Fibroblast growth factor 4(FGF-4) is a heparin binding member of the FGF family. Mature human FGF4 shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell selfrenewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease. |
*本产品仅供科研试验用，不可直接用于人体。
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