| 产品名称 | Human ThermoStable bFGF |
|---|---|
| 中文名称 | 重组热稳定型人碱性成纤维细胞生长因子 |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | >98% as determined by SDS-PAGE |
| 内毒素 | <0.1 EU per μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 1.95 ng/mL |
| 基因编号 | 2247 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P09038/entry |
| 别名 | FGF-2; Heparin-binding growth factor 2; Basic fibroblast growth factor; FGFB; HBGF-2; FGF2; Fibroblast Growth Factor 2 (Basic) |
| 蛋白标签 | N-His |
| 预测分子量 | The recombinant Human ThermoStable bFGF protein consists of 158 amino acids and predicts molecular mass of 17.3 kDa. |
| 产品形式 | Lyophilized from sterile 150 mM NaCl 20 mM Tris , pH 7.5. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human ThermoStable bFGF protein in sterile ddH2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature |
| 储存条件 | Store at -21°C |
| 产品背景 | Thermostable basic fibroblast growth factor (Thermostable bFGF) mutant protein is a thermostable engineered variant of FGF‑2 (bFGF). It originates from the mutant constructed by Dvorak et al. in 2018, which carries 9 amino acid substitutions in wild-type FGF‑2, and has been further optimized on this foundation. Following engineering, the functional half-life of the protein is prolonged from less than 10 hours for wild-type bFGF to over 7 days. While retaining full biological functions, thermostable bFGF possesses greatly improved stability for practical applications relative to wild-type bFGF. In a 2-day medium-change culture regime, this protein can sustain cell growth, pluripotency and differentiation potential. |
*本产品仅供科研试验用，不可直接用于人体。
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