| 产品名称 | Human EGF Protein |
|---|---|
| 中文名称 | 重组人表皮生长因子 |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | > 96 % as determined by SDS-PAGE |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.004 ng/mL. The specific activity of recombinant Human EGF is approximately >2.5 x 108 IU/mg. |
| 基因编号 | 1950 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P01133/entry |
| 别名 | Pro-epidermal growth factor; EGF; Epidermal growth factor; Urogastrone; |
| 蛋白序列 | NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.35 kDa as estimated in SDS-PAGE under reducing conditions. |
| 产品形式 | Lyophilized from sterile 150 mM NaCl 20 mM Tris , pH 8.0. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant human EGF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20℃ |
| 产品背景 | Epidermal growth factor (EGF) is a small growth factor containing 53 amino acid residues that promotes the proliferation of mesenchymal and epidermal cells. Mature proteins, much smaller than 53aa, are produced by protein hydrolysis in the proximal EGF domain across the membrane. EGF is well preserved in mammals, and the EGF in mature humans is 70% the same as that in mice and mice. EGF and fibroblast growth factor 2 (fgf-2) induce the proliferation of neural precursor cells isolated from specific parts of the embryonic and adult brain. EGF and somatopodin c supplemental medium were substituted for 5% thrombocytopenic anemia plasma (PPP) and had the ability to inhibit BALB/C-3T3 cell density. The biological activities of EGF include epithelium formation, angiogenesis, inhibition of gastric acid secretion, proliferation of fibroblasts, and colony formation of epidermal cells in culture. |
*本产品仅供科研试验用，不可直接用于人体。
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