| 产品名称 | Rat EGF Protein |
|---|---|
| 中文名称 | 重组大鼠表皮生长因子 |
| 种属 | Rat |
| 表达宿主 | E. coli |
| 纯度 | ≥ 98 % as determined by SDS-PAGE |
| 内毒素 | < 0.1 EU per μg of the protein as determined by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using Balb/3T3. The ED50 for this effect is 0.1098 ng/mL. The specific activity of recombinant Rat EGF is approximately > 9.11×106 IU/mg. |
| 基因编号 | 25313 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P07522/entry |
| 别名 | Pro-epidermal growth factor; EGF; Epidermal growth factor |
| 蛋白序列 | NSNTGCPPSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWWKLR |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Rat EGF consists of 53 amino acids and has a predicted molecular mass of 6.3 kDa. The apparent molecular mass of the Rat EGF protein is approximately 6.3 kDa in SDS-PAGE under reducing conditions . |
| 产品形式 | Lyophilized from sterile 20 mM Tris, 50 mM NaCl pH 9.5. |
| 使用方式 | Always centrifuge tubes before opening . It is recommended to centrifuge at 12000 rpm for 1 minute.Then reconstitute the lyophilized Rat EGF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20℃ |
| 产品背景 | EGF is a single-pass type I membrane protein,containing 8 LDL-receptor class B repeats and 9 EGF-like domains. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival.Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). |
*本产品仅供科研试验用，不可直接用于人体。
暂无文献引用信息