| 产品名称 | Human IFN-γ Protein |
|---|---|
| 中文名称 | 重组人干扰素-γ |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | > 98 % as determined by SDS-PAGE |
| 内毒素 | < 0.1 EU per μg of the protein as determined by the LAL method. |
| 生物活性 | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 168.4 pg/mL. The specific activity of recombinant Human IFN-γ protein is approximately >5.9 x 106 IU/mg. |
| 基因编号 | 3458 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P01579/entry |
| 别名 | Interferon gamma; IFN-gamma; Immune interferon |
| 蛋白序列 | QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRG |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Human IFN-γ protein consists of 144 amino acids and predicts a molecular mass of 16.91 KDa. |
| 产品形式 | Lyophilized from sterile 150 mM NaCl, 20 mM Tris, pH 7.4. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IFN-γ protein in sterile ddH2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | IFN gamma (IFN-γ), also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN-γ has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity. |
*本产品仅供科研试验用，不可直接用于人体。
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