| 产品名称 | Mouse IFN-γ Protein, His Tag |
|---|---|
| 中文名称 | 重组小鼠干扰素-γ，His-标签 |
| 种属 | Mouse |
| 表达宿主 | E. coli |
| 纯度 | > 95 % as determined by SDS-PAGE |
| 内毒素 | <1.0 EUper μg of the protein as determined by the LAL method |
| 生物活性 | Immobilized Mouse IFN-γ protein at 1 μg/mL (100 μl/well) can bind Mouse IFNGR1, The EC50 of Mouse IFN-γ is 144 ng/mL. The specific activity of recombinant Mouse IFN-γ protein is approximately >9.6 x 103 IU/mg. |
| 基因编号 | 15978 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P01580/entry |
| 别名 | Interferon gamma; IFN-gamma |
| 蛋白序列 | QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRG |
| 蛋白标签 | N-His |
| 预测分子量 | The recombinant Mouse IFN-γ protein consists of 154 amino acids wi and predicts a molecular mass of 16.9 kDa |
| 产品形式 | Lyophilized from sterile 150mM NaCl 20mM Tris , pH 8.0. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IFN-γ protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature |
| 储存条件 | Store at -20°C |
| 产品背景 | IFN gamma (IFN-γ), also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN-γ has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity. |
*本产品仅供科研试验用，不可直接用于人体。