| 产品名称 | Rat GM-CSF Protein, His tag |
|---|---|
| 中文名称 | 重组大鼠粒细胞巨噬细胞集落刺激因子，His-标签 |
| 种属 | Rat |
| 表达宿主 | E. coli |
| 纯度 | > 98 % as determined by SDS-PAGE |
| 内毒素 | <1 EU per μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to induce proliferation in FDC-P1 cells with a linear range of 0.195-12.5 ng/mL. The Specific activity of recombinant Rat GM-CSF is approximately > 5.12x106 IU/mg. |
| 基因编号 | 116630 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P48750/entry |
| 别名 | Colony-stimulating factor (CSF) |
| 蛋白序列 | MWLQNLLFLGIVVYSFSAPTRSPNPVTRPWKHVDAIKEALSLLNDMRALENEKNEDVDIISNEFSIQRPTCVQTRLKLYKQGLRGNLTKLNGALTMIASHYQTNCPPTPETDCEIEVTTFEDFIKNLKGFLFDIPFDCWKPVQK |
| 蛋白标签 | C-His |
| 预测分子量 | The recombinant Rat GM-CSF consists of 127 amino acids and predicts a molecular mass of 14.7 kDa. |
| 产品形式 | Lyophilized from 20 mM Tris-HCl, 50 mM NaCl, pH8.0. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines |
*本产品仅供科研试验用，不可直接用于人体。
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