| 产品名称 | Rat GM-CSF Protein |
|---|---|
| 中文名称 | 重组大鼠粒细胞-巨噬细胞集落刺激因子 |
| 种属 | Rat |
| 表达宿主 | E. coli |
| 纯度 | > 99 % as determined by SDS-PAGE |
| 内毒素 | < 1.0 EU per μg of the protein as determined by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using M-NFS-60. The ED50 for this effect is typically 1.069 ng/mL. The specific activity of recombinant Rat GM-CSF is approximately >9.35x 105 IU/mg. |
| 基因编号 | 12981 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P48750/entry |
| 别名 | Colony-stimulating factor (CSF) |
| 蛋白序列 | MWLQNLLFLGIVVYSFSAPTRSPNPVTRPWKHVDAIKEALSLLNDMRALENEKNEDVDIISNEFSIQRPTCVQTRLKLYKQGLRGNLTKLNGALTMIASHYQTNCPPTPETDCEIEVTTFEDFIKNLKGFLFDIPFDCWKPVQK |
| 蛋白标签 | Tag free |
| 预测分子量 | The Recombinant Rat GM-CSF protein consists of 147 amino acids and predicts a molecular mass of 16.9KDa. |
| 产品形式 | Lyophilized from sterile 20 mM Tris with 150 mM NaCl, pH 7.4. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat GM-CSF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent development. Several cell lineages in the reproductive tract and gestational tissues synthesise GM-CSF under direction by ovarian steroid hormones and signalling agents originating in male seminal fluid and the conceptus. The pre-implantation embryo, invading placental trophoblast cells and the abundant populations of leukocytes controlling maternal immune tolerance are all subject to GM-CSF regulation. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 (cellular) immune responses in cognate T cells. The active form of the protein is found extracellularly as a homodimer, and the encoding gene is localized to a related gene cluster at chromosome region 5q31 which is known to be associated with 5q-syndrome and acute myelogenous leukemia. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. While the benefits of GM-CSF in this arena have been promising, recent reports have suggested the potential for GM-CSF to induce immune suppression and, thus, negatively impact outcomes in the management of cancer patients. GM-CSF deficiency in pregnancy adversely impacts fetal and placental development, as well as progeny viability and growth after birth, highlighting this cytokine as a central maternal determinant of pregnancy outcome with clinical relevance in human fertility. |
*本产品仅供科研试验用，不可直接用于人体。
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