| 产品名称 | Mouse G-CSF Protein |
|---|---|
| 中文名称 | 重组小鼠粒细胞集落刺激因子 |
| 种属 | Mouse |
| 表达宿主 | E. coli |
| 纯度 | > 99 % as determined by SDS-PAGE |
| 内毒素 | <1.0 EUper μg of the protein as determined by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using M-NFS-60. The ED50 for this effect is typically 2.038 pg/mL. The specific activity of recombinant Mouse G-CSF is approximately >4.91x 108 IU/mg. |
| 基因编号 | 12985 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P09920/entry |
| 别名 | Mouse G-CSF |
| 蛋白序列 | MAQLSAQRRMKLMALQLLLWQSALWSGREAVPLVTVSALPPSLPLPRSFLLKSLEQVRKIQASGSVLLEQLCATYKLCHPEELVLLGHSLGIPKASLSGCSSQALQQTQCLSQLHSGLCLYQGLLQALSGISPALAPTLDLLQLDVANFATTIWQQMENLGVAPTVQPTQSAMPAFTSAFQRRAGGVLAISYLQGFLETARLALHHLA |
| 蛋白标签 | Tag free |
| 预测分子量 | The Recombinant Mouse G-CSF protein consists of 178 amino acids and predicts a molecular mass of 18.95 KDa |
| 产品形式 | Lyophilized from sterile 20 mM Tris with 150 mM NaCl, pH 7.4. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse G-CSF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature |
| 储存条件 | Store at -20°C |
| 产品背景 | Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction. |
*本产品仅供科研试验用，不可直接用于人体。
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