Human TNF-α Protein

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Human TNF-α Protein - SDS-PAGE电泳图
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10μg 50μg 100μg 1mg
货号： CY124
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产品名称 Human TNF-α Protein
中文名称 重组人肿瘤坏死因子-α
种属 Human
表达宿主 E. coli
纯度 > 95 % as determined by SDS-PAGE
内毒素 < 0.1 EU per μg of the protein as determined by the LAL method.
生物活性 Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1.747 pg/mL. The specific activity of recombinant Human TNF-α is approximately 5.7 x 108 IU/mg.
基因编号 7124
蛋白链接 https://www.uniprot.org/uniprotkb/P01375/entry
别名 Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 (TNF-a)
蛋白序列 MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
蛋白标签 Tag free
预测分子量 The recombinant Human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa.
产品形式 Lyophilized from sterile 150 mM NaCl 20 mM Tris , pH 8.0.
使用方式 Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human TNF-α protein in sterile ddH2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
运输方式 The product is shipped at ambient temperature.
储存条件 Store at -20°C
产品背景 Tumor necrosis factor alpha (TNF-α), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-α. TNF-α protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-α is involved in fighting against the tumorigenesis, thus is regarded as a molecular insight in cancer treatment.

*本产品仅供科研试验用，不可直接用于人体。