| 产品名称 | Mouse TNF-α Protein |
|---|---|
| 中文名称 | 重组小鼠肿瘤坏死因子-α |
| 种属 | Mouse |
| 表达宿主 | E. coli |
| 纯度 | >95% as determined by SDS-PAGE |
| 内毒素 | < 0.1 EU per μg of the protein as determined by the LAL method. |
| 生物活性 | Measured in a cytotoxicity assay using L929 cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 10.95 pg/mL. The specific activity of recombinant Mouse TNF-α is approximately >9.1 x 107 IU/mg. |
| 基因编号 | 21926 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P06804/entry |
| 别名 | Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 (TNF-a) |
| 蛋白序列 | MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Mouse TNF-α protein consists of 157 amino acids and has a predicted molecular mass of 17 KDa. |
| 产品形式 | Lyophilized from sterile PBS, pH 7.4. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse TNF-α protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. |
*本产品仅供科研试验用，不可直接用于人体。
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