| 产品名称 | Rat TNF-α Protein |
|---|---|
| 中文名称 | 重组大鼠肿瘤坏死因子-α |
| 种属 | Rat |
| 表达宿主 | E. coli |
| 纯度 | >98% as determined by SDS-PAGE |
| 内毒素 | < 0.1 EU per µg of the protein as determined by the LAL method. |
| 生物活性 | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1.208 pg/mL. The specific activity of recombinant Rat TNF-α is approximately >8.228 x 108 IU/mg. |
| 基因编号 | 24835 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P16599/entry |
| 别名 | Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 (TNF-a) |
| 蛋白序列 | MSTESMIRDVELAEEALPKKMGGLQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPNKEEKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPKYLDITESGQVYFGVIAL |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Rat TNF-α consists of 156 amino acids and has a predicted molecular mass of 17.2 kDa. |
| 产品形式 | Lyophilized from sterile 20 mM tris 150 mM NaCl, pH 8.0. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat TNF-α protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Tumor necrosis factor α (TNF-α), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-α. TNF-α protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-α is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. |
*本产品仅供科研试验用，不可直接用于人体。
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