| 产品名称 | Human LIF Protein |
|---|---|
| 中文名称 | 重组人白血病抑制因子 |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | > 98 % as determined by SDS-PAGE |
| 内毒素 | < 0.1 EU per μg of the protein by the LALmethod |
| 生物活性 | Measured in a cell proliferation assay using TF -1. The ED50 for this effect is typically 0.137 ng/mL. The specific activity of recombinant Human LIF is approximately >7.30 x 106 IU/mg. |
| 基因编号 | 2668 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P15018/entry |
| 别名 | Differentiation-stimulating factor (D factor) Melanoma-derived LPL inhibitor (MLPLI) |
| 蛋白序列 | SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Human LIF Protein consists of 180 amino acids and predicts a molecular mass of 22.1 kDa |
| 产品形式 | Lyophilized from sterile 20 mM Tris, 50 mM NaCl, pH8.0 |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Leukemia Inhibitory Factor (LIF) belongs to the IL-6 superfamily of cytokines. LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines. Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways. LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro. Exercise- induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy. LIF also negatively regulates Th2 and Th17 cell differentiation |
*本产品仅供科研试验用，不可直接用于人体。
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