| 产品名称 | Human RANKL Protein |
|---|---|
| 中文名称 | 重组人可溶性RANK配体 |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | ≥98% as determined by SDS-PAGE |
| 内毒素 | <0.1 EU per μg of the protein as determined by the LAL method. |
| 生物活性 | Immobilized human sRANKL protein at 1μg/mL (100μl/well) can bind Human OPG (C-Fc), The EC50 of Human OPG is 188 ng/mL. The specific activity of recombinant Human sRANKL protein is approximately 5.32 x 103 IU/mg. |
| 基因编号 | 8600 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/O14788/entry |
| 别名 | Osteoclast differentiation factor (ODF) Osteoprotegerin ligand (OPGL) Receptor activator of nuclear factor kappa-B ligand (RANKL) TNF-related activation-induced cytokine (TRANCE) |
| 蛋白序列 | MRRASRDYTKYLRGSEEMGGGPGAPHEGPLHAPPPPAPHQPPAASRSMFVALLGLGLGQVVCSVALFFYFRAQMDPNRISEDGTHCIYRILRLHENADFQDTTLESQDTKLIPDSCRRIKQAFQGAVQKELQHIVGSQHIRAEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Human sRANKL consists of 175 amino acids(N-Met) and has a predicted molecular mass of 19 kDa. The apparent molecular mass of the Human sRAKL protein is approximately 19 kDa in SDS-PAGE under reducing conditions. |
| 产品形式 | Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.0. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to centrifuge at 12,000 rpm for 1 minute. It is recommended to reconstitute the lyophilized Human sRANKL protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. TNFSF11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation. |
*本产品仅供科研试验用，不可直接用于人体。
暂无文献引用信息