| 产品名称 | Human BMP-4 Protein |
|---|---|
| 中文名称 | 重组人骨形态发生蛋白 4 |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | >95 % as determined by SDS-PAGE |
| 内毒素 | < 0.1 EU per μg of the protein as determined by the LAL method. |
| 生物活性 | Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is 1.205 ng/mL. As shown in the figure below, the number of positive cells increased as the concentration of BMP4 protein was added, and this effect was measured by the TRAP kit. |
| 基因编号 | 652 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P12644/entry |
| 别名 | Bone morphogenetic protein 2B; BMP-2B |
| 蛋白序列 | MIPGNRMLMVVLLCQVLLGGASHASLIPETGKKKVAEIQGHAGGRRSGQSHELLRDFEATLLQMFGLRRRPQPSKSAVIPDYMRDLYRLQSGEEEEEQIHSTGLEYPERPASRANTVRSFHHEEHLENIPGTSENSAFRFLFNLSSIPENEVISSAELRLFREQVDQGPDWERGFHRINIYEVMKPPAEVVPGHLITRLLDTRLVHHNVTRWETFDVSPAVLRWTREKQPNYGLAIEVTHLHQTRTHQGQHVRISRSLPQGSGNWAQLRPLLVTFGHDGRGHALTRRRRAKRSPKHHSQRARKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Human BMP-4 consists of 106 amino acids and predicts a molecular mass of 11.9 kDa. |
| 产品形式 | Lyophilized from sterile 20 mM Tris, 50 mM NaCl, pH 8.0. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human BMP-4 protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Bone morphogenetic protein -4 (BMP-4) is one of nine structurally related BMP's, belonging to transforming growth factor-β (TGF-β) superfamily, which secretes proteins. Mature BMP-4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. BMP-4 treatment promoted the proliferation of HCC cells and induced autophagy. Mechanism study shows that autophagy induced by BMP-4 is mediated by activating JNK1/Bcl2 pathway. JNK1 inhibitor and JNK1 knockout can weaken BMP-4-induced autophagy and eliminate BMP-4-promoted HCC cell growth. BMP-4 promotes the proliferation of HCC through autophagy activation of JNK1/Bcl-2 signaling. |
*本产品仅供科研试验用，不可直接用于人体。
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