| 产品名称 | Human GDNF Protein |
|---|---|
| 中文名称 | 重组人胶质细胞源性神经营养因子 |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | > 98% as determined by SDS-PAGE |
| 内毒素 | < 0.1 EU per μg of the protein as determined by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using SH-SY5Y. The ED50 for this effect is typically 11.33 ng/mL. The specific activity of recombinant Human GDNF is approximately >8.83 x 104 IU/mg. |
| 基因编号 | 4254 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P39905/entry |
| 别名 | Astrocyte-derived trophic factor (ATF) |
| 蛋白序列 | MKLWDVVAVCLVLLHTASAFPLPAGKRPPEAPAEDRSLGRRRAPFALSSDSNMPEDYPDQFDDVMDFIQATIKRLKRSPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Human GDNF consists of 134 amino acids and predicts a molecular mass of 15.1 kDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions. |
| 产品形式 | Lyophilized from sterile 20 mM Tris, 150mM NaCl, pH7.4. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. |
*本产品仅供科研试验用，不可直接用于人体。
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