| 产品名称 | Human Noggin Protein, His tag |
|---|---|
| 中文名称 | 重组人Noggin蛋白，His-标签 |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | > 98% as determined by SDS-PAGE |
| 内毒素 | <0.1 EU per μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to inhibit the BMP4 protein to induce ATDC5 cells to produce alkaline phosphatase. The ED50 of this effect is 1.22 ng/mL. The specific activity of recombinant Human Noggin is approximately >8.20x 105 IU/mg. As shown in the figure below, the number of positive cells decreases with increasing Noggin protein concentration, an effect measured by the TRAP kit. |
| 基因编号 | 9241 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/Q13253/entry |
| 别名 | Noggin |
| 蛋白序列 | MERCPSLGVTLYALVVVLGLRATPAGGQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC |
| 蛋白标签 | C-His |
| 预测分子量 | The recombinant Human Noggin Protein consists of 206 amino acids and predicts a molecular mass of 23.2 kDa. |
| 产品形式 | Lyophilized from sterile 20 mM Tris, 50 mM NaCl, pH8.0. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | The secreted polypeptide Noggin, encoded by the Noggin gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, Noggin may have a principal role in creating morphogenic gradients. Noggin appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of Noggin suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as Noggin. All Noggin mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of human Noggin is highly homologous to that of Xenopus, rat and mouse. |
*本产品仅供科研试验用，不可直接用于人体。
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