| 产品名称 | Mouse IL-4 Protein |
|---|---|
| 中文名称 | 重组小鼠白介素-4 |
| 种属 | Mouse |
| 表达宿主 | E. coli |
| 纯度 | > 95 % as determined by SDS-PAGE |
| 内毒素 | <1 EU per μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using NFS-60. The ED50 for this effect is typically 5.37 ng/mL. The specific activity of recombinant Mouse IL4 is approximately >1.86 x 105 IU/mg. |
| 基因编号 | 16189 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P07750/entry |
| 别名 | B-cell lgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1 (BSF-1); lGG1 induction factor; Lymphocyte stimulatory factor 1 |
| 蛋白序列 | MGLNPQLVVILLFFLECTRSHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Mouse IL4 consists of 119 amino acids and predicts a molecular mass of 13.4 KDa. |
| 产品形式 | Lyophilized from sterile PBS, pH 7.4. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL4 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells. |
*本产品仅供科研试验用，不可直接用于人体。
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