| 产品名称 | Human Sonic Hedgehog Protein |
|---|---|
| 中文名称 | 重组人音猬因子（Shh） |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | > 95 % as determined by SDS-PAGE |
| 内毒素 | <1 EU per μg of the protein by the LAL method. |
| 生物活性 | Measure by its ability to induce alkaline phosphatase production by C3H10T1/2 cells. The ED₅₀ for this effect is <10 ng/mL. As shown in the figure below, the number of positive cells increased as the concentration of SHH protein was added, and this effect was measured by the Alkaline Phosphatase Assay Kit. |
| 基因编号 | 6469 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/Q15465/entry |
| 别名 | HHG1, HLP3, HPE3, MCOPCB5, SMMCI, ShhNC, TPT, TPTPS |
| 蛋白序列 | IIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant human SHH (aa 1-197) consists of 185 amino acids and has a predicted molecular mass of 21 kDa. |
| 产品形式 | Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.4. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IFN-γ protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Sonic HedgeHog, also known as sonic hedgehog protein, belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine, liver, lung, and kidney. Sonic HedgeHog is a protein that is vital in guiding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5, triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3. |
*本产品仅供科研试验用，不可直接用于人体。
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