| 产品名称 | Human DKK-1 Protein |
|---|---|
| 中文名称 | 重组人Dickkopf相关蛋白1（DKK-1） |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | > 95 % as determined by SDS-PAGE |
| 内毒素 | <1 EU per μg of the protein by the LAL method. |
| 生物活性 | Measure by its ability to induce alkaline phosphatase production by C3H10T1/2 cells. The ED₅₀ for this effect is 1.011 ng/mL. As shown in the figure below, the number of positive cells increased as the concentration of DKK-1 protein was reduced, and this effect was measured by the Alkaline Phosphatase Assay kit. |
| 基因编号 | 22943 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/O94907/entry |
| 别名 | DKK-1, SK |
| 蛋白序列 | TLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant human DKK1 consists of 473 amino acids and predicts a molecular mass of 52.5 kDa. |
| 产品形式 | Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.4. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IFN-γ protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers. |
*本产品仅供科研试验用，不可直接用于人体。
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