| 产品名称 | Human SCF Protein |
|---|---|
| 中文名称 | 重组人干细胞因子（SCF） |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | > 95 % as determined by SDS-PAGE |
| 内毒素 | <1 EU per μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using TF-1 Cell. The ED50 for this effect is typically 0.026 ng/mL. The specific activity of recombinant Human SCF is approximately >3.85 x 107 IU/mg. |
| 基因编号 | 4254 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P21583/entry |
| 别名 | DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SCF, SF, SHEP7, SLF, WS2F |
| 蛋白序列 | EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVAASSLRNDSSSSNRKAKNPPGDSSLHWAAMALPALFSLIIGFAFGALYWKKRQPSLTRAVENIQINEEDNEISMLQEKEREFQEV |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant Human KITLG consists of 165 amino acids and predicts a molecular mass of 18.6 kDa. |
| 产品形式 | Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.4. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IFN-γ protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| 运输方式 | The product is shipped at ambient temperature. |
| 储存条件 | Store at -20°C |
| 产品背景 | Similar to Kit ligand precursor (C-kit ligand), also known as Stem cell factor (SCF), Mast cell growth factor (MGF), or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein can augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone. |
*本产品仅供科研试验用，不可直接用于人体。
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