| 产品名称 | Mouse β-NGF protein |
|---|---|
| 中文名称 | 重组小鼠神经营养因子 |
| 种属 | Mouse |
| 表达宿主 | E. coli |
| 纯度 | > 95 % as determined by SDS-PAGE |
| 内毒素 | <1 EU per μg of the protein by the LAL method. |
| 生物活性 | Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.03ng/mL. The specific activity of recombinant Mouse β-NGF is > 3.33 x 107U/mg. |
| 基因编号 | 18049 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P01139/entry |
| 别名 | Beta-NGF; Beta-Nerve Growth Factor; NGF; NGFB |
| 蛋白序列 | MSSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG |
| 蛋白标签 | Tag free |
| 预测分子量 | The recombinant protein has a calculated MW of 14.41 kDa. |
| 产品形式 | Liquid in from sterile 20 mM Tris, 150 mM NaCl, pH 8.0, 5% Glycerol,1 mM EDTA. |
| 使用方式 | Always centrifuge tubes before opening. It is recommended to diluted the protein using the PBS buffer or Protein storage solution to working concentration. |
| 运输方式 | The product is shipped at gel pack with blue ice. |
| 储存条件 | Store at -20°C |
| 产品背景 | Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of Rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and Rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. |
*本产品仅供科研试验用，不可直接用于人体。
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