| 产品名称 | Human IL-10 Protein (Animal-Free) |
|---|---|
| 中文名称 | 重组人白介素10（无动物源） |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | >98% as determined by SDS-PAGE. |
| 内毒素 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measure by its ability to induce MC/9‑2 cells proliferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human IL-10 is approximately >1x106 IU/ mg. |
| 基因编号 | 3586 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P22301 |
| 别名 | interleukin 10, B-TCGF, CSIF, TGIF, IL10 |
| 蛋白序列 | MSPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN |
| 蛋白标签 | C-His |
| 预测分子量 | The protein has a calculated MW of 19.6 kDa.The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis). |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| 使用方式 | 1. Before opening, centrifuge at 3000 rpm for 5 mins. 2. Initial Reconstitution · Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL. · Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. · Store at 2°C to 8°C for up to 1 week. 3. Extended Storage · After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml. · Prepare aliquots (≥20 μl). · Store at -20°C or -80°C for 3 to 6 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | The product is shipped at Blue Ice |
| 储存条件 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 产品背景 | Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. Many different types of cells can produce IL-10, including immune cells and non-immune cells. IL-10 exerts inhibitory functions on DCs and macrophages, which is a potent inhibitor of antigen presentation and limit the production of the Th1-associated cytokines IL-2 and interferon-γ (IFN-γ). IL-10 is also a key immunoregulator during infection due to its inhibitory effect on inflammatory cytokine production. Consequently, the excessive Th1 and CD8+ T cell responses could be suppressed during infection. |
*本产品仅供科研试验用，不可直接用于人体。
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