| 产品名称 | Human IL-15 Protein (Animal-Free) |
|---|---|
| 中文名称 | 重组人白介素15（无动物源） |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | >95% as determined by SDS-PAGE. |
| 内毒素 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measure by its ability to induce proliferation in CTLL-2 cells. The ED₅₀ for this effect is < 3 ng/mL. The specific activity of recombinant human IL-15 is > 2 x 106 IU/mg. |
| 基因编号 | 3600 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P40933 |
| 别名 | interleukin 15, IL-T, IL15 |
| 蛋白序列 | NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFIN TS |
| 蛋白标签 | N-His |
| 预测分子量 | The protein has a calculated MW of 13.7 kDa.The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis). |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing NaPi buffer, 0.018% SDS, pH 7.5. If you have any concerns or special requirements, please confirm with us. |
| 使用方式 | 1. Before opening, centrifuge at 3000 rpm for 5 mins. 2. Initial Reconstitution · Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL. · Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. · Store at 2°C to 8°C for up to 1 week. 3. Extended Storage · After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml. · Prepare aliquots (≥20 μl). · Store at -20°C or -80°C for 3 to 6 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | The product is shipped at Blue Ice |
| 储存条件 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 产品背景 | Interleukin-15 (IL-15) is a 14-15 kDa glycoprotein with immune regulatory functions in many diverse cell types. IL-15 can be constitutively expressed in a variety of cell types stored as intracellular protein in the cytoplasm as well as transport to the cell surface, while only secreted from some cell types including monocytes, dendritic cells, epithelial cells, bone marrow stromal cells, and fibroblasts. As a pleiotropic cytokine, IL-15 mediates the crosstalk between innate immunity and adaptive immunity whose principal role is to kill virally infected cells. IL-15 plays a crucial role in the development, differentiation, and survival of NK cells. In monocytes, IL-15 induces the production of IL-8 and monocyte chemotactic protein 1 (MCP-1), which recruits neutrophils and monocytes to sites of infection. IL-15 can also act as a chemo-attractant in T lymphocytes and regulate the differentiation of T lymphocytes. |
*本产品仅供科研试验用，不可直接用于人体。
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