| 产品名称 | Human FGF-4 Protein (Animal-Free) |
|---|---|
| 中文名称 | 重组人成纤维细胞生长因子4（无动物源） |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | >95% as determined by SDS-PAGE. |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2.5 ng/mL. The specific activity of recombinant human FGF-4 is > 4 x 105 IU/mg. |
| 基因编号 | 2249 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P08620 |
| 别名 | fibroblast growth factor 4, HST-1, Transforming protein KS3, HBGF-4, FGF4 |
| 蛋白序列 | MGRGGAAAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL with polyhistidine tag at the C-terminus |
| 蛋白标签 | C-His |
| 预测分子量 | The protein has a calculated MW of 20.70 kDa.The protein migrates as 22 kDa under reducing condition (SDS-PAGE analysis). |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| 使用方式 | 1. Before opening, centrifuge at 3000 rpm for 5 mins. 2. Initial Reconstitution · Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL. · Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. · Store at 2°C to 8°C for up to 1 week. 3. Extended Storage · After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml. · Prepare aliquots (≥20 μl). · Store at -20°C or -80°C for 3 to 6 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | The product is shipped at Blue Ice |
| 储存条件 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 产品背景 | Fibroblast Growth Factors-4 (FGF-4) is a 22 kDa member of the fibroblast Growth Factors with 206 amino acid residues. FGF-4 can regulate embryonic development, cell proliferation, and cell differentiation. FGF-4 is an important role development during embryogenesis. |
*本产品仅供科研试验用，不可直接用于人体。
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