Human/Mouse/Rat Activin A Protein (Animal-Free)
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| 产品名称 | Human/Mouse/Rat Activin A Protein (Animal-Free) |
|---|---|
| 中文名称 | 重组人/小鼠/大鼠激活素A（无动物源） |
| 种属 | Human |
| 表达宿主 | E. coli |
| 纯度 | >95% as determined by SDS-PAGE. |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measure by its ability to inhibit the proliferation of mouse MPC-11 cells. The ED₅₀ for this effect is <10 ng/mL. The specific activity of recombinant human Activin A is approximately >1.0 x 103 IU/mg. |
| 基因编号 | 3624 |
| 蛋白链接 | https://www.uniprot.org/uniprotkb/P08476 |
| 别名 | Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein |
| 蛋白序列 | GLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS |
| 蛋白标签 | Tag Free |
| 预测分子量 | The protein has a calculated MW of 13.1 kDa.The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis). |
| 产品形式 | The protein was lyophilized from a 0.2 µm filtered solution containing 50 mM Tris, 500 mM NaCl, pH 8.5. If you have any concerns or special requirements, please confirm with us. |
| 使用方式 | 1. Before opening, centrifuge at 3000 rpm for 5 mins. 2. Initial Reconstitution · Reconstitute the Lyophilized Protein in sterile H₂O to a concentration of 100-200 μg/mL. · Then, incubate it at room temperature for at least 20 mins to ensure sufficient dissolution. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. · Store at 2°C to 8°C for up to 1 week. 3. Extended Storage · After the initial reconstitution, further dilute the reconstituted protein in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml. · Prepare aliquots (≥20 μl). · Store at -20°C or -80°C for 3 to 6 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | The product is shipped at Blue Ice |
| 储存条件 | Lyophilized Protein: Store at -20°C for 1 year. Initial Reconstitution: Store at 2°C to 8°C for up to 1 week. Extended Storage: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 产品背景 | Activin is the member of the TGF beta superfamily of cytokines. The current understanding of Activin A is classified as a hormone, a Growth Factors, and a cytokine. Depending on the different cell type, that overexpression of Activin A can either inhibit or promote cells division. There are important implications for tumor biology. Activin A also increase joint inflammation in rheumatoid arthritis (RA) and induces pathogenic mechanisms in the respiratory system. |
*本产品仅供科研试验用，不可直接用于人体。
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